The chromatography of L-myosin on diethylaminoethylcellulose.

at pH 5.0 run slowly through, when aminoacetone is retained and ALA runs out. After the column has been washed with water the aminoacetone is eluted with HCl. For subsequent chromatography the eluate is evaporated to dryness in vacuo, the aminoacetone hydrochloride extracted with absolute ethanol and the bulky precipitate of NaCl filtered off. The solution is then sufficiently salt-free for most purposes. Paper chromatography. Chromatographic separation of ALA and aminoacetone can be achieved by using the following solvent system: ethanol-Macetic acid-pyridine-water (95:10:3:3, by vol.) in which ALA and aminoacetone have RF values of 0-15 and 0-3 respectively. In five acidic solvents tested, including butanol-acetic acid, ALA and aminoacetone had identical B. values. In view of this, and particularly since alkaline solvents cannot be used, the two compounds could easily be confused. Both give the same colour reactions with ninhydrin (yellow, turning purple at room temperature). ALA and aminoacetone can be separated by paper electrophoresis at pH 4-0-65 and the two can also be distinguished chromatographically by first converting them into pyrroles (Mauzerall & Granick, 1956). SUMMARY